HANDS-ON WORKSHOP IN 3D-printing & Microfluidics for BIOENGINEERING

May 9-11th 2018

Course Content
Keynote Speaker
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The 10th annual Hand's on Workshop in Micro and Nano Bioengineering will be held from Wednesday, May 9th to Friday, May 11th at McGill Univeristy in Montréal, Québec. Participants will learn the foundations of micro and nano biotechnologies and gain first hand experience with Computer Aided Design (CAD), 3D printing, soft lithography, microfluidics, immunoassays and more. This year we will be also adding to the workshop segements in 3D bioprinting and tissue engineering. Last year we had almost 30 attendees from industry and academia partaking in the workshop and we look forward to having you join us this year!

The course is open to students, scientists, engineers, and industry professionals in any area of research who would like to learn more about micro and nanoscience technology in general. The course is of particular interest to biomedical researchers, chemists, physicists, as well as biomedical, mechanical, chemical, and materials engineers.

Course Content

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The workshop lecture series will begin with an introduction and overview of micro and nano biotechnology and applications. This will be followed by more in depth lectures on 1) Microfluidics and applications in biomedical technologies, 2) Additive Manufacturing and Rapid Prototyping, and 3) 3D Bioprinting in tissue engineering. The purpose of these lectures is to give participants an overview of the current trends and applications of micro and nano biotechnologies in a number of fields.


Participants will first learn the basics of Computer Aided Design (CAD) software (AutoCAD). Design rules and limitations will be discussed for high resolution 3D printing. In the interactive portion of the session, participants will design and make adjustments to a CAD model for a 3D printed microfluidic device with bioengineering applications. Computers are provided; however, participants are encouraged to bring their own laptops.


3D Printing
Participants will be introduced to the benefits and shortcomings of rapid prototyping in microfluidic applications using a 3D printer with 50 micrometer resolution. You will get to see the printing process for your devices (designed during module 2) and take them home.
3D Bioprinting: Human Tissues on Demand with Next Generation 3D Bioprinting
Aspect Biosystems' unique Lab-on-a-Printer™ technology merges cutting-edge advancements in 3D printing, microfluidics and biomaterials to recreate the structure and complexity necessary to build functional human tissues. In this workshop, you will get an introduction to the RX1 Bioprinting platform and explore the considerations for designing and printing functional 3D tissues. We will also be bioprinting relevant tissue structures that can be broadly applied to various tissue engineering applications.
Microfluidics: Miniaturized Mosaic Immunoassays
Using this assay, many different antigens can be screened at the same time in a small area using a minute amount of reagents, making the assays less labour-, time- and cost- intensive. You will learn how to handle microfluidic devices that operate based on capillary effects only (no pumps, no plugs), and that can be serviced manually using conventional micropipettes. You will learn how to use microfluidics to pattern lines of antibodies onto a surface to carry out combinatorial miniaturized mosaic immunoassay.
Cell-Laden Hydrogel Patterning
In this experiment, a 3D-printed stamp is used to print cells in hydrogels onto cell culture substrates. The stamp, made of photocurable resin, is obtained by stereolithography-based 3D printing. Once printed, the stamp can be used to pick up and deposit small droplets of hydrogels containing cells onto a surface. Multiple cell types can be patterned onto the same surface using different stamps to generate cell co-cultures. After patterning, the hydrogels are cross-linked into place to perform the co-culture experiment.


In this session, presentations will be given by participants who will show how Nano/Microtechnology can be applied to their research area. This session is a highlight of the course as it helps participants to relate what they just learned to their work, and at the same time providing a snapshot of the breadth of micro and nanotechnologies. There will also be an opportunity to participate in a process design challenge and compete for the best presentation award this year! To conclude the day, past year participants will be invited back to talk about their current research using the tools and techniques they learned through this workshop.


The workshop is comprised of 4 modules:

  • Lecture series
  • CAD Module
  • Lab Module
  • Design Challenge

The workshop ends with a keynote lecture from a distinguished speaker and a fun wine and cheese (and beer) social -- don't miss it! 


Keynote Speaker


Miniaturizing 3D-Printed Microfluidics: Status and Opportunities

While there is great interest in 3D printing for microfluidic device fabrication, the challenge has been to achieve feature sizes that are in the truly microfluidic regime (<100 μm). The fundamental problem is that commercial tools and materials, which excel in many other application areas, have not been developed to address the unique needs of microfluidic device fabrication. Consequently, in my group we have created our own stereolithographic 3D printer and materials that are specifically tailored to meet these needs. We show that flow channels as small as 18 μm x 20 μm can be reliably fabricated, as well as compact active elements such as valves and pumps. With these capabilities, we demonstrate highly integrated 3D printed microfluidic devices that measure only a few millimeters on a side, and that integrate to separate chip-to-world interfaces through high density interconnects (up to 88 interconnects per square mm) that are directly 3D printed as part of a device chip. These advances open the door to 3D printing as a replacement for expensive cleanroom fabrication processes, with the additional advantage of fast (30 minute), parallel fabrication of many devices in a single print run due to their small size.



Samuel Sia, Columbia University

"Microfluidics for Personal Mobile Diagnostics"



Milicia Radisic, University of Toronto

"Towards Person-on-a-Plate: Microfabrication and Biodegradable Polymers for High Fidelity Modelling of Human Tissues"

Keynote 2015

Axel Guenther, University of Toronto

"Blood Vessels-on-a-Chip and Microfluidic Bioprinting"



The workshop is located at McGill University in the heart of downtown Montréal, Québec. Module 1 Lectures and the Design Challenge of the workshop will be held in the Strathcona Anatomy and Dentistry building, Room M48 (3640 Rue University). Module 2 (CAD Design Studio) will be held in the McConnell Engineering Building (3480 Rue University), Room 10. Module 3 (Lab Session) will be held in the Genome Québec and McGill Innovation Centre (740 Dr. Penfield Ave), Room 6500. The keynote speaker will be in room of M1 of the Strathcona Anatomy and Dentistry building (3640 rue University). The wine and cheese following the keynote will be held on the fifth floor of the Genome Centre (740 Docteur-Penfield Ave). 

Coming from out of town? There are plenty of hotels within walking distance to McGill campus. Montréal bus routes 24, 144, and 80 will also take you near campus if staying further away. If you have any questions about transportation or accommodations, just send us an email at junckerlab.workshop@gmail.com.



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The Hands-on Workshop in Micro & Nano Bioengineering is made possible through the generous support of our sponsors. To find out more about becoming a sponsor, please email us at junckerlab.workshop@gmail.com.